Structural evidence for recognition of a single epitope by two distinct antibodies

Proteins. 2000 Sep 1;40(4):572-8.

Abstract

The structure of a complex between the hemagglutinin of influenza virus and the Fab of a neutralizing antibody was determined by X-ray crystallography at 2.8 A resolution. This antibody and another which has only 56% sequence identity bind to the same epitope with very similar affinities and in the same orientation. One third of the interactions is conserved in the two complexes; a significant proportion of the interactions that differ are established by residues of the H3 complementarity-determining regions (CDR) which adopt distinct conformations in the two antibodies. This demonstrates that there is a definite flexibility in the selection of antibodies that bind to a given epitope, despite the high affinity of their complexes. This flexibility allows the humoral immune response to be redundant, a feature that may be useful in achieving longer lasting protection against evolving viral pathogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies / chemistry*
  • Antigen-Antibody Complex / chemistry
  • Crystallography, X-Ray
  • Epitopes*
  • Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
  • Immunoglobulin Fab Fragments / chemistry
  • Models, Molecular
  • Molecular Sequence Data

Substances

  • Antibodies
  • Antigen-Antibody Complex
  • Epitopes
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Immunoglobulin Fab Fragments