Temperature-dependent functional expression of a plant K(+) channel in mammalian cells

I Szabò; A Negro; P M Downey; M Zoratti; F Lo Schiavo; G M Giacometti

doi:10.1006/bbrc.2000.3095

Temperature-dependent functional expression of a plant K(+) channel in mammalian cells

Biochem Biophys Res Commun. 2000 Jul 21;274(1):130-5. doi: 10.1006/bbrc.2000.3095.

Authors

I Szabò¹, A Negro, P M Downey, M Zoratti, F Lo Schiavo, G M Giacometti

Affiliation

¹ Department of Biology, CRIBI, CNR Unit for Biomembranes, University of Padua, Via G. Colombo 3, Padua, 35121, Italy. [email protected]

PMID: 10903907
DOI: 10.1006/bbrc.2000.3095

Abstract

The Arabidopsis thaliana potassium channel KAT1 was expressed and characterized in Chinese hamster ovary cells. KAT1-GFP fusion protein was successfully targeted to the plasma membrane and electrophysiological analysis revealed functional expression of KAT1 only in cells cultured at 30 degrees C. The main biophysical characteristics of KAT1 are similar to those described for the channel expressed in other systems. CHO cells represent an advantageous expression system and may be the system of choice to study the expression, assembly, function, and regulation of plant potassium channels in general.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arabidopsis / metabolism
Arabidopsis Proteins
CHO Cells
Cell Membrane / metabolism
Cricetinae
Electrophysiology
Green Fluorescent Proteins
Luminescent Proteins / metabolism
Microscopy, Fluorescence
Plant Proteins
Potassium Channels / biosynthesis*
Potassium Channels, Inwardly Rectifying*
Recombinant Fusion Proteins / metabolism
Temperature*
Transfection

Substances

Arabidopsis Proteins
KAT1 protein, Arabidopsis
Luminescent Proteins
Plant Proteins
Potassium Channels
Potassium Channels, Inwardly Rectifying
Recombinant Fusion Proteins
Green Fluorescent Proteins