[The effect of Epstein-Barr virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cells]

Zhonghua Zhong Liu Za Zhi. 1998 Jul;20(4):245-7.
[Article in Chinese]

Abstract

Objective: To investigate the effect of EB virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cell.

Methods: Expression vector for BHRF1 was constructed and transfected into nsaopharyngeal carcinoma cell line, CNE2. The alteration of biologic behaviour of the transfected cells was tested after 60Co radiation.

Results: BHRF1 expression could inhibit the expression of proliferative cell nuclear antigen, redistribute cell cycle, decrease cell sensitivity to radiation, and enhance their survival ability.

Conclusion: It is suggested that BHRF1 expression can increase the apoptotic resistance induced by radiation.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis* / radiation effects
  • Cell Cycle / radiation effects
  • Cobalt Radioisotopes
  • Gene Expression
  • Genes, Viral
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Mice
  • Mice, Nude
  • Nasopharyngeal Neoplasms / metabolism
  • Nasopharyngeal Neoplasms / pathology*
  • Proliferating Cell Nuclear Antigen / metabolism
  • Transfection
  • Tumor Cells, Cultured / metabolism
  • Viral Proteins / biosynthesis*

Substances

  • BHRF1 protein, Human herpesvirus 4
  • Cobalt Radioisotopes
  • Proliferating Cell Nuclear Antigen
  • Viral Proteins