[Northern blot analysis of nm23 gene expression in human lung cancer]

Zhonghua Zhong Liu Za Zhi. 1998 Sep;20(5):342-4.
[Article in Chinese]

Abstract

Objective: To investigate the role of nm23 gene expression in lung cancer.

Methods: Forty human lung cancer tissues and 19 non-cancerous pulmonary tissues were studied for their nm23-H1 and nm23-H2 mRNA expression with nonradioactive Northern blot hybridization. Correlation of nm23 mRNA expression with clinical features of lung cancer was analyzed.

Results: The mRNA expression of nm23-H2 gene in poorly differentiated squamous-cell carcinoma was significantly decreased compared to that in moderately differentiated squamous-cell carcinoma. The mRNA expression of nm23-H1 and nm23-H2 genes in small cell lung cancer was decreased compared to that in squamous-cell carcinoma. No significant difference in nm23 mRNA expression was observed in lung cancers with and without lymph node metastasis, nor was there significant difference in patients with lung cancers in different stages.

Conclusion: The mRNA expression of nm23 gene is correlated with the degree of differentiation of lung cancer, but there is no evidence of metastasis suppression effect by nm23 gene.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism
  • Blotting, Northern
  • Carcinoma, Small Cell / genetics
  • Carcinoma, Small Cell / metabolism*
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism*
  • Gene Expression
  • Humans
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism*
  • Monomeric GTP-Binding Proteins / biosynthesis*
  • Monomeric GTP-Binding Proteins / genetics
  • NM23 Nucleoside Diphosphate Kinases
  • Nucleoside-Diphosphate Kinase*
  • RNA, Messenger / genetics
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics

Substances

  • NM23 Nucleoside Diphosphate Kinases
  • RNA, Messenger
  • Transcription Factors
  • NME1 protein, human
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins