Subunits of neurosteroid sulfatase from bovine brain

I H Park; B K Han; J H Baek; Y W Ryu; Y H Park; D H Jo

doi:10.1016/s0960-0760(00)00065-0

Subunits of neurosteroid sulfatase from bovine brain

J Steroid Biochem Mol Biol. 2000 Jun;73(3-4):135-9. doi: 10.1016/s0960-0760(00)00065-0.

Authors

I H Park¹, B K Han, J H Baek, Y W Ryu, Y H Park, D H Jo

Affiliation

¹ Brain Disease Research Center, School of Medicine, Ajou University, Suwon, South Korea.

PMID: 10925212
DOI: 10.1016/s0960-0760(00)00065-0

Abstract

We have purified the neurosteroid sulfatase (NSS) from Triton X-100 solubilized microsomes of bovine brain about 100-fold. The purified enzyme is composed of two catalytic units (MW: 57 kDa) and two regulatory units (MW: 38 kDa), making it an alpha(2)beta(2) heterotetramer, whose apparent molecular weight was 180 kDa by gel filtration in the presence of Triton X-100.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arylsulfatases / chemistry
Arylsulfatases / isolation & purification
Arylsulfatases / metabolism*
Brain / enzymology*
Cattle
Chromatography, DEAE-Cellulose
Electrophoresis, Polyacrylamide Gel
Female
Microsomes / enzymology
Steryl-Sulfatase

Substances

Arylsulfatases
Steryl-Sulfatase