T-cell migration into allografts

J Exp Med. 1975 May 1;141(5):1210-5. doi: 10.1084/jem.141.5.1210.

Abstract

The ability of T and B lymphocytes to migrate into skin allografts undergoing rejection was studied in mice. Spleen cells from CBA/J mice sensitized to transplantation antigens of A/J or C57BL/6 mice were separated on immunabsorbent columns into purified populations of T and B cells, labeled in vitro with 3H-uridine and injected intravenously into CBA/J mice with 7-day old skin iso and allografts (A/J or C57BL/6). The mice were sacrificed 24 h later and studied by autoradiography. After transfer of either unfractionated spleen cells or T cells, large numbers of labeled cells were found in the cellular infiltrate of allografts, whereas extremely few were seen in isografts. In contrast, after transfer of B cells, almost no labeled cells were detected either in the allografts or the isografts, although they, like T cells, homed normally to lymphoid tissue.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • B-Lymphocytes / immunology*
  • Cell Movement
  • Graft Rejection*
  • Immunization, Passive*
  • Male
  • Mice
  • Mice, Inbred Strains
  • Skin Transplantation*
  • Spleen / cytology
  • T-Lymphocytes / immunology*
  • Transplantation Immunology
  • Transplantation, Homologous
  • Tritium
  • Uridine

Substances

  • Tritium
  • Uridine