CD34+ selected cells in mismatched stem cell transplantation: a single centre experience of haploidentical peripheral blood stem cell transplantation

Bone Marrow Transplant. 2000 May:25 Suppl 2:S9-11. doi: 10.1038/sj.bmt.1702344.

Abstract

Over the past 3 years we have performed 10 haploidentical peripheral blood stem cell transplants in patients with incurable haematological malignancies and no prospect of a matched unrelated donor within an adequate time period. Conditioning consisted of ATG, TBI, thiotepa, cyclophosphamide and additional radioimmunotherapy in five patients. All patients received G-CSF mobilized peripheral blood stem cell grafts. GVHD prophylaxis consisted of T cell depletion by CD34+ selection; no post-transplant immunosuppression was given in nine patients. Stable engraftment was achieved in nine patients; one case of acute graft rejection was observed. Seven patients developed grade I acute GVHD, and six patients have developed chronic GVHD. Infections were the most significant clinical problem post transplant. Two patients have suffered a relapse of their disease and two further patients have died of transplant-related complications. After a median follow-up of 13 months (range 5-37 months) six patients are surviving in remission. We conclude that haploidentical PBSCT is a reasonable alternative to a MUD transplant.

MeSH terms

  • Adult
  • Antigens, CD34 / metabolism*
  • Cytomegalovirus Infections / etiology
  • Disease-Free Survival
  • Female
  • Graft Survival
  • Graft vs Host Disease / etiology
  • Haplotypes
  • Hematopoietic Stem Cell Transplantation / adverse effects
  • Hematopoietic Stem Cell Transplantation / methods*
  • Humans
  • Leukemia / immunology
  • Leukemia / therapy
  • Male
  • Middle Aged
  • Recurrence

Substances

  • Antigens, CD34