Abstract
To elucidate the role of E-cadherin in matrix metalloproteinases (MMPs) expression, we transfected to squamous carcinoma cells with E-cadherin cDNA. HN5 cells and mock-transfected HN5-neo cells expressed proMMP-2 and active MMP-2. E-cadherin-transfected HN5-EC cells produced comparable proMMP-2 but low active MMP-2; and membrane type 1-MMP (MT1-MMP) mRNA declined. Phosphorylated ERK, a marker of mitogen-activated protein (MAP) kinase cascade, also declined in HN5-EC cells. The addition of anti-E-cadherin antibody resulted in the disappearance of these alterations in HN5-EC cells. These results suggest that E-cadherin suppresses MAP kinase cascade and down-regulates MT1-MMP.
MeSH terms
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Blotting, Western
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Cadherins / genetics
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Cadherins / metabolism*
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Carcinoma, Squamous Cell / enzymology
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Carcinoma, Squamous Cell / metabolism*
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Cytoskeletal Proteins / metabolism
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DNA Primers
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DNA, Complementary / metabolism
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Down-Regulation
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Fluorescent Antibody Technique
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Gene Expression Regulation, Neoplastic
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Humans
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MAP Kinase Signaling System*
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Matrix Metalloproteinase 1 / biosynthesis
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Matrix Metalloproteinase 1 / genetics
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Matrix Metalloproteinase 1 / metabolism*
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Matrix Metalloproteinase 2 / biosynthesis
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Matrix Metalloproteinase 2 / genetics
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Matrix Metalloproteinase 2 / metabolism*
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Mitogen-Activated Protein Kinases / metabolism*
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Precipitin Tests
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Tissue Inhibitor of Metalloproteinase-2 / metabolism
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Trans-Activators*
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Transfection
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Tumor Cells, Cultured
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alpha Catenin
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beta Catenin
Substances
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CTNNA1 protein, human
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CTNNB1 protein, human
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Cadherins
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Cytoskeletal Proteins
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DNA Primers
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DNA, Complementary
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RNA, Messenger
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Trans-Activators
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alpha Catenin
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beta Catenin
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Tissue Inhibitor of Metalloproteinase-2
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Mitogen-Activated Protein Kinases
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 1