cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana

Mol Plant Microbe Interact. 2000 Aug;13(8):895-8. doi: 10.1094/MPMI.2000.13.8.895.

Abstract

To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography. Most differential gene fragments were derived from P. parasitica.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / microbiology*
  • Base Sequence
  • DNA Primers
  • DNA, Complementary
  • Gene Expression Regulation, Fungal
  • Gene Expression Regulation, Plant
  • Genes, Fungal*
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques*
  • Oomycetes / genetics*

Substances

  • DNA Primers
  • DNA, Complementary

Associated data

  • GENBANK/AW737063
  • GENBANK/AW737064
  • GENBANK/AW737065
  • GENBANK/AW737066
  • GENBANK/AW737067
  • GENBANK/AW737068
  • GENBANK/AW737069
  • GENBANK/AW737070
  • GENBANK/AW737071
  • GENBANK/AW737072
  • GENBANK/AW737073
  • GENBANK/AW737074
  • GENBANK/AW737075
  • GENBANK/AW737076
  • GENBANK/AW737077