A beta fibrillogenesis: kinetic parameters for fibril formation from congo red binding

J Struct Biol. 2000 Jun;130(2-3):123-9. doi: 10.1006/jsbi.2000.4258.

Abstract

Using Scatchard analysis, we have formulated as a function of time and pH the relationship between the binding of Congo red to Alzheimer's beta-amyloid and the aggregation number (i.e., monomer concentration within fibrils) as defined by nucleation-dependent self-assembly. This provides a basis on which to determine the kinetic parameters for fibril formation from the observed concentration of bound Congo red.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amyloid beta-Peptides / chemistry*
  • Amyloid beta-Peptides / metabolism*
  • Amyloid beta-Peptides / ultrastructure
  • Congo Red / chemistry
  • Congo Red / metabolism
  • Dimerization
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • Models, Chemical
  • Time Factors

Substances

  • Amyloid beta-Peptides
  • Congo Red