New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol)

J Chromatogr B Biomed Sci Appl. 2000 Jun 23;743(1-2):79-84. doi: 10.1016/s0378-4347(99)00516-2.

Abstract

The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Molecular Weight
  • Polyethylene Glycols / chemistry*
  • Resins, Plant / chemistry*
  • Trees / chemistry*
  • Water / chemistry

Substances

  • Resins, Plant
  • Water
  • Polyethylene Glycols