Methyl-chrysanthemate is one of the intermediates of pyrethroid type insecticides. The acute toxicity of the test item was investigated in rats after single oral, dermal and inhalation applications. The irritation effect was determined by Draize method. Buehler method was applied to evaluate the sensitization potential of the test item. The mutagenic effect was assessed on Salmonella typhimurium strains. Furthermore metaphase chromosome aberration assay was performed on CHO cell line to check the structural chromosome aberrations.