Abstract
We have raised an anti-idiotypic antibody against the cell surface IgM of the murine BCL1 tumor cells. This antiserum reacts exclusively with the IgM expressed on the tumor cells and detects a unique population of cells in the spleen and blood of the tumor-bearing mice. When these cells are stimulated in vitro with LPS, they secrete an IgM bearing the same idiotype as the cell surface Ig. These results are discussed in terms of a model for the immunotherapy of a chronic lymphocytic leukemia-like syndrome in mice.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocytes / immunology*
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Clone Cells / immunology
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Fluorescent Antibody Technique
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Immunoglobulin Idiotypes*
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Immunoglobulin M / immunology
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Immunoglobulin lambda-Chains / immunology
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Leukemia, Experimental / immunology*
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Mice
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Mice, Inbred BALB C
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Myeloma Proteins / immunology
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Rabbits
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Receptors, Antigen, B-Cell / immunology
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Time Factors
Substances
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Immunoglobulin Idiotypes
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Immunoglobulin M
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Immunoglobulin lambda-Chains
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Myeloma Proteins
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Receptors, Antigen, B-Cell