A precolumn derivatization high-performance liquid chromatographic method with improved sensitivity and specificity for the determination of astragaloside IV in Radix Astragali

J Chromatogr Sci. 2000 Aug;38(8):325-8. doi: 10.1093/chromsci/38.8.325.

Abstract

A reversed-phase high-performance liquid chromatographic method is developed for the determination of astragaloside (AGS) IV, which is known as the active constituent of Radix Astragali. The method uses precolumn derivatization with benzoyl chloride to form the benzoyl ester of AGS IV quantitatively and is carried out with a wide-ranging concentration (0.004-0.080 mg/mL) of the derivatized AGS IV. The eluent consists of 90% methanol, 4% tetrahydrofuran, 6% water, and 0.2% triethylamine, with vitamin D3 added as the internal standard.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Drugs, Chinese Herbal / analysis*
  • Glucosides / analysis*
  • Glucosides / chemistry
  • Molecular Structure
  • Plant Roots / chemistry*
  • Saponins / analysis*
  • Saponins / chemistry
  • Sensitivity and Specificity
  • Spectrometry, Mass, Fast Atom Bombardment
  • Triterpenes*

Substances

  • Drugs, Chinese Herbal
  • Glucosides
  • Saponins
  • Triterpenes
  • astragaloside A