Evaluation of a capture screening enzyme-linked immunosorbent assay for combined determination of immunoglobulin M and G antibodies produced during Dengue infection

S Kit Lam; C Lan Ew; J L Mitchell; A J Cuzzubbo; P L Devine

doi:10.1128/CDLI.7.5.850-852.2000

Evaluation of a capture screening enzyme-linked immunosorbent assay for combined determination of immunoglobulin M and G antibodies produced during Dengue infection

Clin Diagn Lab Immunol. 2000 Sep;7(5):850-2. doi: 10.1128/CDLI.7.5.850-852.2000.

Authors

S Kit Lam¹, C Lan Ew, J L Mitchell, A J Cuzzubbo, P L Devine

Affiliation

¹ WHO Collaborating Centre for Arbovirus Reference and Research (Dengue and Dengue Haemorrhagic Fever), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. [email protected]

Abstract

A commercially available enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Screening ELISA) that utilized both immunoglobulin M (IgM) and IgG capture in the same microtiter well for the diagnosis of dengue infection was evaluated. Sensitivity in primary and secondary dengue was 95%, while specificity was 94%.

MeSH terms

Antibodies, Viral / blood*
Antibodies, Viral / immunology
Dengue / blood
Dengue / diagnosis*
Dengue / immunology
Enzyme-Linked Immunosorbent Assay / methods
Evaluation Studies as Topic
Humans
Immunoglobulin G / blood*
Immunoglobulin G / immunology
Immunoglobulin M / blood*
Immunoglobulin M / immunology
Reagent Kits, Diagnostic
Sensitivity and Specificity

Substances

Antibodies, Viral
Immunoglobulin G
Immunoglobulin M
Reagent Kits, Diagnostic