Abstract
Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220(+)BP-1(+) pre-B cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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B-Lymphocytes / drug effects*
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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Cytokines / chemistry
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Cytokines / genetics
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Cytokines / pharmacology*
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DNA, Complementary / isolation & purification
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Female
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Hematopoiesis / drug effects*
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Interleukin-7 / pharmacology
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Male
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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RNA, Messenger / analysis
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / pharmacology
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Cytokines
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DNA, Complementary
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Interleukin-7
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RNA, Messenger
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Recombinant Proteins