Abstract
Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reactive clones were found to encode the dimerization domain of TRF2, a mammalian telomeric protein that binds to duplex TTAGGG repeats at chromosome ends. This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2. The transfected TFR2 colocalized with Ku70.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Nuclear*
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Base Sequence
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Binding Sites
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COS Cells
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DNA Helicases*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Dimerization
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Fluorescent Antibody Technique
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Humans
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Ku Autoantigen
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Precipitin Tests
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Protein Binding
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Repetitive Sequences, Amino Acid
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Saccharomyces cerevisiae Proteins*
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Telomere / genetics
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Telomere / metabolism
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Telomeric Repeat Binding Protein 2
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Transfection
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Two-Hybrid System Techniques
Substances
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Antigens, Nuclear
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DNA-Binding Proteins
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Nuclear Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Telomeric Repeat Binding Protein 2
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high affinity DNA-binding factor, S cerevisiae
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DNA Helicases
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XRCC5 protein, human
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Xrcc6 protein, human
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Ku Autoantigen