Production of recombinant mammalian holo-E2 and E3 and reconstitution of functional branched-chain alpha-keto acid dehydrogenase complex with recombinant E1

Methods Enzymol. 2000:324:192-200. doi: 10.1016/s0076-6879(00)24231-6.

Authors

J L Chuang¹, J R Davie, R M Wynn, D T Chuang

Affiliation

¹ Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas 75390-9038, USA.

PMID: 10989430
DOI: 10.1016/s0076-6879(00)24231-6

No abstract available

MeSH terms

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Animals
Cattle
Escherichia coli / genetics
Humans
Ketone Oxidoreductases / biosynthesis*
Ketone Oxidoreductases / genetics
Ketone Oxidoreductases / metabolism
Multienzyme Complexes / biosynthesis*
Multienzyme Complexes / genetics
Multienzyme Complexes / metabolism
Recombinant Proteins / biosynthesis
Recombinant Proteins / genetics
Recombinant Proteins / metabolism

Substances

Multienzyme Complexes
Recombinant Proteins
Ketone Oxidoreductases
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)