Abstract
The effects of water-soluble chitosan oligomers (WSCO) on the synthesis of nitric oxide (NO) by murine peritoneal macrophages and on macrophage-mediated cytotoxicity towards murine fibrosarcoma Meth A cells were investigated. WSCO alone had no effect on NO synthesis and killing of tumor cells. However, treatment of macrophages with a combination of WSCO and interferon-gamma (IFN-gamma) synergically increased NO synthesis and enhanced killing of tumor cells. The synergism between IFN-gamma and WSCO in NO synthesis and tumoricidal activity was mainly dependent on increased secretion of tumor necrosis factor-alpha by WSCO.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / pharmacology
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Chitin / analogs & derivatives*
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Chitin / pharmacology*
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Chitosan
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Culture Media, Conditioned / pharmacology
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Cytotoxicity, Immunologic / drug effects*
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Dose-Response Relationship, Drug
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Drug Synergism
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Interferon-gamma / pharmacology*
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Macrophages, Peritoneal / drug effects*
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Macrophages, Peritoneal / immunology
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Macrophages, Peritoneal / metabolism
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Mice
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Mice, Inbred C57BL
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Nitric Oxide / biosynthesis*
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Nitric Oxide Synthase / drug effects
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Nitric Oxide Synthase / metabolism
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Nitric Oxide Synthase Type II
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Solubility
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Tumor Cells, Cultured / cytology
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Tumor Cells, Cultured / drug effects
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Tumor Necrosis Factor-alpha / immunology
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Antibodies, Monoclonal
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Culture Media, Conditioned
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Tumor Necrosis Factor-alpha
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Chitin
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Nitric Oxide
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Interferon-gamma
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Chitosan
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse