Abstract
A cDNA encoding an RNA binding protein has been isolated from a cDNA library prepared from larvae of the ascidian Ciona intestinalis. The putative protein of 162 amino acids contained in the N-terminal region one copy of the consensus sequence RNA binding domain and in the C-terminal region a glycine-rich domain. The in vitro translated protein bound various RNA homopolymers, preferentially polyU, polyA, and polyG, and the binding was affected by increasing ionic strength. Northern blot analysis revealed a single transcript of about 0.7 kb in length that was present during embryonic development with two major peaks of accumulation at gastrula and larval stages. Whole-mount in situ hybridization experiments on embryos at different stages of development showed gene expression mainly in mesenchymal cells and in neural tissue.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Ciona intestinalis / embryology*
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Ciona intestinalis / genetics*
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Cloning, Molecular
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Gene Expression Profiling
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Gene Expression Regulation, Developmental*
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In Situ Hybridization
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Molecular Sequence Data
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Oligoribonucleotides / genetics
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Oligoribonucleotides / metabolism
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Organ Specificity
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Protein Binding
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Protein Structure, Tertiary
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RNA, Antisense / genetics
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RNA, Antisense / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Oligoribonucleotides
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RNA binding protein, Ciona intestinalis
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RNA, Antisense
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RNA, Messenger
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RNA-Binding Proteins