Effects of intrauterine IL-6 and IL-8 on the expression of surfactant apoprotein mRNAs in the fetal rat lung

Eur J Obstet Gynecol Reprod Biol. 2000 Nov;93(1):97-103. doi: 10.1016/s0301-2115(00)00247-5.

Abstract

Objective: The purpose of this study was to investigate the effect of interleukin-6 (IL-6) and interleukin-8 (IL-8), whose concentrations are elevated with chorioamnionitis, on the expression of surfactant apoprotein mRNAs in fetal rat lung.

Study design: We employed an animal model in which we were able to administer substances continuously into the cavity between the fetal membranes and endometrium using a miniosmotic pump. Lipopolysaccharide (LPS), IL-6, or IL-8 was administered to timed pregnant rats for 3 days (day 16-19), and fetal lung expression of surfactant apoprotein mRNAs for SP (surfactant apoprotein)-A, SP-B, and SP-C was evaluated by Northern blot hybridization.

Results: Continuous administration of LPS increased the expression of each surfactant apoprotein mRNA, but the expression of mRNAs was not dose-dependent. On the other hand, continuous IL-6 or IL-8 administration increased the expression of each surfactant apoprotein mRNA in a dose-dependent manner.

Conclusion: Fetal lung maturation may be promoted by either IL-6 or IL-8 produced in response to chorioamnionitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Female
  • Gene Expression*
  • Humans
  • Interleukin-6 / administration & dosage*
  • Interleukin-6 / pharmacology
  • Interleukin-8 / administration & dosage*
  • Interleukin-8 / pharmacology
  • Lipopolysaccharides / administration & dosage
  • Lipopolysaccharides / pharmacology
  • Lung / embryology*
  • Lung / metabolism
  • Pregnancy
  • Proteolipids / genetics
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants / genetics*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Interleukin-6
  • Interleukin-8
  • Lipopolysaccharides
  • Proteolipids
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants
  • RNA, Messenger