Abstract
Membrane-enveloped vesicles travel among the compartments of the cytoplasm of eukaryotic cells, delivering their specific cargo to programmed locations by membrane fusion. The pairing of vesicle v-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) with target membrane t-SNAREs has a central role in intracellular membrane fusion. We have tested all of the potential v-SNAREs encoded in the yeast genome for their capacity to trigger fusion by partnering with t-SNAREs that mark the Golgi, the vacuole and the plasma membrane. Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Biological Transport
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Cell Compartmentation*
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Endoplasmic Reticulum / metabolism
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Escherichia coli
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Fungal Proteins / metabolism
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Golgi Apparatus / metabolism
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Intracellular Membranes / metabolism*
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Liposomes
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Membrane Fusion / physiology*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Membrane Proteins / physiology*
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Qa-SNARE Proteins
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Qc-SNARE Proteins
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Recombinant Proteins / metabolism
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SNARE Proteins
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Saccharomyces cerevisiae
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Saccharomyces cerevisiae Proteins*
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Vesicular Transport Proteins*
Substances
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Fungal Proteins
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Liposomes
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Membrane Proteins
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Qa-SNARE Proteins
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Qc-SNARE Proteins
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Recombinant Proteins
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SEC9 protein, S cerevisiae
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SNARE Proteins
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SSO1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Vesicular Transport Proteins