Abstract
Regulation of eukaryotic gene expression requires ATP-dependent chromatin remodeling enzymes, such as SWI/SNF, and histone acetyltransferases, such as Gcn5p. Here we show that SWI/SNF remodeling controls recruitment of Gcn5p HAT activity to many genes in late mitosis and that these chromatin remodeling enzymes play a role in regulating mitotic exit. In contrast, interphase expression of GAL1, HIS3, PHO5, and PHO8 is accompanied by SWI/SNF-independent recruitment of Gcn5p HAT activity. Surprisingly, prearresting cells in late mitosis imposes a requirement for SWI/SNF in recruiting Gcn5p HAT activity to the GAL1 promoter, and GAL1 expression also becomes dependent on both chromatin remodeling enzymes. We propose that SWI/SNF and Gcn5p are globally required for mitotic gene expression due to the condensed state of mitotic chromatin.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylation
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Acetyltransferases / genetics
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Acetyltransferases / metabolism
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Anaphase / drug effects
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Cell Cycle Proteins*
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Chromatin / chemistry
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Chromatin / enzymology
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Chromatin / genetics
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Chromatin / metabolism*
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DNA-Binding Proteins*
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Genes, Fungal / genetics
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Histone Acetyltransferases
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Histones / chemistry
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Histones / metabolism
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Interphase / drug effects
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Mitosis* / drug effects
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Mitosis* / genetics
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Nocodazole / pharmacology
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Nucleic Acid Conformation
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Phenotype
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Promoter Regions, Genetic / genetics
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Protein Kinases / chemistry
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein Structure, Tertiary
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Saccharomyces cerevisiae Proteins*
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Saccharomyces cerevisiae* / cytology
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Saccharomyces cerevisiae* / enzymology
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Saccharomyces cerevisiae* / genetics
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Saccharomyces cerevisiae* / metabolism
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Time Factors
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Cell Cycle Proteins
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Chromatin
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DNA-Binding Proteins
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Fungal Proteins
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Histones
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SWI5 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Acetyltransferases
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Histone Acetyltransferases
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Protein Kinases
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SNF1-related protein kinases
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Protein Serine-Threonine Kinases
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Nocodazole