Cleavage of yeast tRNAPhe with complementary oligonucleotide conjugated to a small ribonuclease mimic

N G Beloglazova; V N Sil'nikov; M A Zenkova; V V Vlassov

doi:10.1016/s0014-5793(00)02029-9

Cleavage of yeast tRNAPhe with complementary oligonucleotide conjugated to a small ribonuclease mimic

FEBS Lett. 2000 Sep 22;481(3):277-80. doi: 10.1016/s0014-5793(00)02029-9.

Authors

N G Beloglazova¹, V N Sil'nikov, M A Zenkova, V V Vlassov

Affiliation

¹ Institute of Bioorganic Chemistry, Siberian Branch of Russian Academy of Sciences, 8 Lavrentiev Avenue, 630090, Novosibirsk, Russia.

PMID: 11007978
DOI: 10.1016/s0014-5793(00)02029-9

Abstract

An oligonucleotide conjugate bearing a chemical construct mimicking the catalytic center of ribonuclease A has been designed and studied. The conjugate efficiently cleaves yeast tRNAPhe at a single site adjacent to the target complementary sequence.

MeSH terms

Anthracenes / chemistry
Base Sequence
Catalysis / drug effects
Hydrolysis / drug effects
Imidazoles / chemistry
Molecular Mimicry*
Molecular Sequence Data
Oligonucleotides, Antisense / chemical synthesis
Oligonucleotides, Antisense / chemistry*
RNA, Complementary / chemistry*
RNA, Fungal / chemistry
RNA, Transfer, Phe / antagonists & inhibitors
RNA, Transfer, Phe / chemistry*
Ribonucleases / chemistry*
Saccharomyces cerevisiae
Thionucleotides / chemistry

Substances

9-(aminomethyl)-9,10-dihydroanthracene
Anthracenes
Imidazoles
Oligonucleotides, Antisense
RNA, Complementary
RNA, Fungal
RNA, Transfer, Phe
Thionucleotides
Ribonucleases