Detection and quantitation of human immunodeficiency virus type 1 in the female genital tract. The Division of AIDS Treatment Research Initiative 009 Study Group

J Clin Microbiol. 2000 Oct;38(10):3822-4. doi: 10.1128/JCM.38.10.3822-3824.2000.

Abstract

Human immunodeficiency virus type 1 (HIV-1) was detected in the genital tracts of 59% of 225 women by RNA PCR and in 7% of the women by culture. In a comparison of two sampling methods, endocervical swabs were more sensitive than cervicovaginal lavage for HIV-1 RNA detection by PCR but not by culture and their sensitivity was independent of the concentration of HIV-1 RNA.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cross-Sectional Studies
  • Female
  • HIV Infections / virology*
  • HIV-1 / isolation & purification*
  • Humans
  • Polymerase Chain Reaction / methods
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Vaginal Smears / methods*

Substances

  • RNA, Viral