Abstract
Here we describe development of transgenic elite rice lines expressing a Bt fusion gene derived from cryIA(b) and cryIA(c) under the control of rice actinI promoter. The lines used in the study were indica CMS restorer line of Minghui 63 and its derived hybrid rice Shanyou 63. The level of Bt fusion protein CryIA(b)/CryIA(c) detected in Minghui 63 (T51-1) plants was 20 ng/mg soluble protein. The Bt Shanyou 63 was field-tested in natural and repeated heavy manual infestation of two lepidopteran insects, leaffolder and yellow stem borer. The transgenic hybrid plants showed high protection against both insect pests without reduced yield.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / genetics
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Animals
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Bacillus thuringiensis Toxins
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Bacillus thuringiensis*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Bacterial Toxins*
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China
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Endotoxins / genetics
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Endotoxins / metabolism*
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Gene Expression Regulation, Plant
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Genetic Engineering*
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Hemolysin Proteins
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Homozygote
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Hybridization, Genetic
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Insecticides / metabolism*
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Lepidoptera / physiology
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Oryza / genetics*
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Oryza / growth & development*
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Oryza / parasitology
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Pest Control, Biological
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Phenotype
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Plants, Genetically Modified
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Promoter Regions, Genetic / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transgenes / genetics
Substances
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Actins
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Bacterial Toxins
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Endotoxins
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Hemolysin Proteins
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Insecticides
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Recombinant Fusion Proteins
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insecticidal crystal protein, Bacillus Thuringiensis