Multiple levels of regulation specify the polarity of an asymmetric cell division in C. elegans

Development. 2000 Nov;127(21):4587-98. doi: 10.1242/dev.127.21.4587.

Abstract

Wnt signaling systems play important roles in the generation of cell and tissue polarity during development. We describe a Wnt signaling system that acts in a new way to orient the polarity of an epidermal cell division in C. elegans. In this system, the EGL-20/Wnt signal acts in a permissive fashion to polarize the asymmetric division of a cell called V5. EGL-20 regulates this polarization by counteracting lateral signals from neighboring cells that would otherwise reverse the polarity of the V5 cell division. Our findings indicate that this lateral signaling pathway also involves Wnt pathway components. Overexpression of EGL-20 disrupts both the asymmetry and polarity of lateral epidermal cell divisions all along the anteroposterior (A/P) body axis. Together our findings suggest that multiple, inter-related Wnt signaling systems may act together to polarize asymmetric cell divisions in this tissue.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Body Patterning
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development*
  • Caenorhabditis elegans Proteins*
  • Cell Communication
  • Cell Division
  • Cell Polarity
  • Epidermal Cells
  • Epidermis / physiology
  • Gene Expression Regulation, Developmental
  • Genes, Homeobox*
  • Genotype
  • Glycoproteins / genetics
  • Glycoproteins / physiology
  • Helminth Proteins / genetics
  • Helminth Proteins / physiology
  • Larva
  • Mitogens / physiology
  • Mutagenesis
  • Proto-Oncogene Proteins / metabolism
  • Signal Transduction
  • Wnt Proteins
  • Zebrafish Proteins*

Substances

  • Caenorhabditis elegans Proteins
  • Egl-20 protein, C elegans
  • Glycoproteins
  • Helminth Proteins
  • Mitogens
  • Proto-Oncogene Proteins
  • Wnt Proteins
  • Zebrafish Proteins