Metabolic labeling of glycoproteins with chemical tags through unnatural sialic acid biosynthesis

Methods Enzymol. 2000:327:260-75. doi: 10.1016/s0076-6879(00)27282-0.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biochemistry / methods*
  • Cell Line
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Glycoproteins / chemistry*
  • Glycoproteins / metabolism*
  • HeLa Cells
  • Hexosamines / antagonists & inhibitors
  • Hexosamines / biosynthesis
  • Hexosamines / pharmacology
  • Humans
  • Jurkat Cells
  • Ketones / metabolism
  • Microscopy, Fluorescence
  • Models, Biological
  • N-Acetylneuraminic Acid / biosynthesis
  • N-Acetylneuraminic Acid / chemistry*
  • N-Acetylneuraminic Acid / metabolism*
  • Substrate Specificity
  • Time Factors

Substances

  • Glycoproteins
  • Hexosamines
  • Ketones
  • N-levulinoyl mannosamine
  • N-Acetylneuraminic Acid
  • N-acetylmannosamine