Marked differences in quantity of infectious human immunodeficiency virus type 1 detected in persons with controlled plasma viremia by a simple enhanced culture method

J Clin Microbiol. 2000 Nov;38(11):4246-8. doi: 10.1128/JCM.38.11.4246-4248.2000.

Abstract

Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus detected. This method provides a simple and reproducible means for monitoring cell-associated HIV-1.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • CD4-Positive T-Lymphocytes / physiology
  • CD4-Positive T-Lymphocytes / virology*
  • Cells, Cultured
  • HIV Core Protein p24 / analysis
  • HIV Infections / virology*
  • HIV-1 / isolation & purification*
  • HIV-1 / physiology*
  • Humans
  • RNA, Viral / blood
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Viral Load
  • Viremia / virology
  • Virus Cultivation

Substances

  • HIV Core Protein p24
  • RNA, Viral