Abstract
Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus detected. This method provides a simple and reproducible means for monitoring cell-associated HIV-1.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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CD4-Positive T-Lymphocytes / physiology
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CD4-Positive T-Lymphocytes / virology*
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Cells, Cultured
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HIV Core Protein p24 / analysis
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HIV Infections / virology*
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HIV-1 / isolation & purification*
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HIV-1 / physiology*
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Humans
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RNA, Viral / blood
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Reproducibility of Results
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Sensitivity and Specificity
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Viral Load
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Viremia / virology
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Virus Cultivation
Substances
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HIV Core Protein p24
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RNA, Viral