Abstract
Tax protein expressed by human T cell leukemia virus type 1 (HTLV-1) is a strong trans-activator of its own LTR promoter; it also affects the function of multiple cellular genes involved in cell cycle control and transcription. One way in which Tax exerts its pleiotropic effects is through protein-protein interaction with cellular cofactors. By using yeast two-hybrid technology, we have isolated several cellular proteins that bind to Tax. Two of these are MAD1, a mitotic checkpoint control protein, and TXBP151, a suppressor of tumor necrosis factor alpha-induced apoptosis. Here we discuss findings describing the role of MAD1 in exit of cells from mitosis and TXBP151 in NF-kappaB activation.
MeSH terms
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Carrier Proteins / metabolism
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Cell Cycle / physiology
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Cell Cycle Proteins
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Cell Line
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Gene Products, tax / genetics*
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Gene Products, tax / metabolism*
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HeLa Cells
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Human T-lymphotropic virus 1 / genetics
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Human T-lymphotropic virus 1 / metabolism*
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Humans
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Intracellular Signaling Peptides and Proteins*
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NF-kappa B / genetics
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NF-kappa B / metabolism*
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Neoplasm Proteins*
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Nuclear Proteins
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Transcriptional Activation
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Two-Hybrid System Techniques
Substances
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Carrier Proteins
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Cell Cycle Proteins
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Gene Products, tax
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Intracellular Signaling Peptides and Proteins
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MAD1L1 protein, human
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NF-kappa B
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Neoplasm Proteins
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Nuclear Proteins
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Phosphoproteins
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Repressor Proteins
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TAX1BP1 protein, human