Abstract
Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that this protein was overexpressed by >/=170% in 9 of 10 clinical isolates of Esherichia coli with high-level ciprofloxacin resistance (MICs, >/=32 microg/ml) but not in any of the 15 isolates for which the MIC was </=1 microg/ml.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Anti-Infective Agents / pharmacology*
-
Bacterial Proteins / biosynthesis*
-
Bacterial Proteins / immunology
-
Bacterial Proteins / physiology
-
Biological Transport / physiology
-
Ciprofloxacin / pharmacology*
-
Drug Resistance, Microbial / physiology
-
Escherichia coli / drug effects*
-
Escherichia coli / metabolism
-
Escherichia coli Proteins*
-
Humans
-
Lipoproteins / biosynthesis*
-
Lipoproteins / immunology
-
Lipoproteins / physiology
-
Membrane Transport Proteins
-
Microbial Sensitivity Tests
Substances
-
AcrA protein, E coli
-
Anti-Infective Agents
-
Bacterial Proteins
-
Escherichia coli Proteins
-
Lipoproteins
-
Membrane Transport Proteins
-
Ciprofloxacin