Abstract
Enterococcus faecium 10/96A from Brazil was resistant to vancomycin (MIC, 256 microg/ml) but gave no amplification products with primers specific for known van genotypes. A 2,368-bp fragment of a van cluster contained one open reading frame encoding a peptide with 83% amino acid identity to VanH(D), and a second encoding a D-alanine-D-lactate ligase with 83 to 85% identity to VanD. The divergent glycopeptide resistance phenotype was designated VanD4.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics*
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Brazil
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Drug Resistance, Microbial / genetics
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Enterococcus faecium / drug effects
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Enterococcus faecium / genetics*
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Genetic Variation
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Genotype
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Glycopeptides
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Humans
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Molecular Sequence Data
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Peptide Synthases*
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Sequence Homology, Amino Acid
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Glycopeptides
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Peptide Synthases
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VanD protein, Enterococcus faecium