Differential expression of tenascin-C, tenascin-R, tenascin/J1, and tenascin-X in spinal cord scar tissue and in the olfactory system

Exp Neurol. 2000 Dec;166(2):350-62. doi: 10.1006/exnr.2000.7543.

Abstract

The members of the tenascin family are involved in a number of developmental processes, mainly by their ability to regulate cell adhesion. We have here studied the distribution of mRNAs for tenascin-X, -C, and -R and the closely related molecule tenascin/J1 in the olfactory system and spinal cord. The olfactory bulb and nasal mucosa were studied during late embryonic and early postnatal development as well as in the adult. The spinal cord was studied during late embryonic development and after mechanical lesions. In the normal rat, the spinal cord and olfactory bulb displayed similar patterns of tenascin expression. Tenascin-C, tenascin-R, and tenascin/J1 were all expressed in the olfactory bulb and spinal cord during development, while tenascin/J1 was the only extensively expressed tenascin molecule in the adult. In both regions tenascin/J1 was expressed in both nonneuronal and neuronal cells. After a spinal cord lesion, mRNAs for tenascin-C, -X, -R, and/J1 were all upregulated and had their own specific spatial and temporal expression patterns. Thus, even if axonal outgrowth occurs to some extent both in the adult rat primary olfactory system and in spinal cord scar tissue after lesion, the tenascin expression patterns in these two situations are totally different.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Cicatrix / metabolism
  • Cicatrix / physiopathology
  • Female
  • Gene Expression Regulation, Developmental
  • Immunohistochemistry
  • In Situ Hybridization
  • Microglia / chemistry
  • Microglia / ultrastructure
  • Microscopy, Immunoelectron
  • Neurons / chemistry
  • Neurons / ultrastructure
  • Olfactory Bulb / chemistry*
  • Olfactory Bulb / embryology
  • RNA, Messenger / analysis
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord / chemistry*
  • Spinal Cord / embryology
  • Spinal Cord / pathology
  • Spinal Cord Injuries / metabolism
  • Spinal Cord Injuries / pathology
  • Spinal Cord Injuries / physiopathology*
  • Tenascin / analysis
  • Tenascin / genetics*

Substances

  • RNA, Messenger
  • Tenascin
  • tenascin X
  • tenascin R