Abstract
We report the characterization of a zebrafish DnaJ-like protein, homologue to the human Hsp40 protein hdj1, that we named zf-Hsp40. We have studied its expression during fin regeneration and we show that zf-Hsp40 mRNA level is drastically increased in all ray segments beneath the regenerated part, or blastema, in response to caudal fin amputation. In order to investigate whether zf-Hsp40 is part of a stress response system after injury, we studied the expression of the zebrafish Hsc70 transcript. Our results show a correlation between the expression of the two transcripts.
MeSH terms
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Amino Acid Sequence
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Animals
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Carrier Proteins / biosynthesis*
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Carrier Proteins / genetics*
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DNA, Complementary / metabolism
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Gene Library
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HSC70 Heat-Shock Proteins
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HSP40 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins*
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Heat-Shock Proteins / biosynthesis*
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Heat-Shock Proteins / genetics*
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In Situ Hybridization
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Molecular Sequence Data
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RNA, Messenger / metabolism
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Regeneration
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Sequence Homology, Amino Acid
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Zebrafish / embryology*
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Zebrafish / physiology*
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Zebrafish Proteins
Substances
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Carrier Proteins
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DNA, Complementary
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HSC70 Heat-Shock Proteins
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HSP40 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins
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Heat-Shock Proteins
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RNA, Messenger
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Zebrafish Proteins
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dnaja protein, zebrafish
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hspa8 protein, zebrafish