De novo acute hepatitis B infection in a previously vaccinated liver transplant recipient due to a strain of HBV with a Met 133 Thr mutation in the "a" determinant

Liver. 2000 Oct;20(5):411-4. doi: 10.1034/j.1600-0676.2000.020005411.x.

Abstract

De novo HBV infection post-liver transplantation (LT) from an anti-HBc seropositive donor rarely presents as acute failure. We report a 42-year-old Caucasian female, HBsAg and anti-HBc seronegative, with primary biliary cirrhosis who received an allograft from a HBsAg negative, anti-HBc seropositive donor. The patient, previously vaccinated years pre-LT, was re-vaccinated against HBV and 1 year post-LT had an anti-HBs titre of 256 IU/l. Two years post-LT, elevated serum aminotransferases and worsening liver function with an INR of 2.0 developed. The HBsAg became positive, anti-HBs undetectable and serum HBV-DNA >2000 pg/ml by hybridisation assay. Liver biopsy revealed significant ballooning degeneration, piecemeal necrosis and positive immunostaining for HBsAg. Progressive liver failure developed followed by sepsis and terminal multi-organ failure. Subsequent analysis of the predominant HBV strain revealed mutations in the "a" determinant: Met 133 Thr (codon change ATG to ACG) and Asn 131 Thr.

Conclusion: ' Acute de novo HBV infection from an anti-HBc sero-positive donor may occur long after LT despite protective anti-HBs titres post-vaccination secondary to the emergence of "a" determinant mutated strains of HBV.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adult
  • Amino Acid Substitution / genetics*
  • Female
  • Hepatitis B / immunology
  • Hepatitis B / pathology*
  • Hepatitis B / virology
  • Hepatitis B Surface Antigens / genetics*
  • Hepatitis B Surface Antigens / immunology
  • Hepatitis B Vaccines / immunology*
  • Hepatitis B virus / genetics
  • Hepatitis B virus / immunology
  • Hepatitis B virus / physiology*
  • Hepatocytes / pathology
  • Hepatocytes / virology
  • Histocytochemistry
  • Humans
  • Liver / pathology
  • Liver / virology
  • Liver Cirrhosis, Biliary / surgery
  • Liver Transplantation* / immunology
  • Mutation / genetics
  • RNA, Viral / blood
  • Sequence Analysis, DNA
  • Tissue Donors
  • Vaccination

Substances

  • Hepatitis B Surface Antigens
  • Hepatitis B Vaccines
  • RNA, Viral