Abstract
WSL-1/TRAMP (DR3) is a member of the tumour necrosis factor (TNF) receptor superfamily which exhibits effects on NF-kappaB activation and apoptosis. TWEAK, a novel TNF-related molecule, has been proposed as the ligand for this receptor. Utilising both human and murine TWEAK ligand, it is shown that TWEAK and WSL-1/TRAMP do not interact in an in vitro binding assay and that TWEAK binds strongly to cells that do not express WSL-1/TRAMP on the cell surface. Biological activity of TWEAK is also observed in these cells. Finally, cells isolated from WSL-1/TRAMP knockout mice are shown to retain their ability to interact with TWEAK. These results suggest that WSL-1/TRAMP is not the major receptor for TWEAK
MeSH terms
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Animals
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Apoptosis
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Apoptosis Regulatory Proteins
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Carrier Proteins / metabolism*
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Cell Line
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Cytokine TWEAK
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Humans
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Interleukin-8 / pharmacology
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Lymphocytes / metabolism
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Mice
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Mice, Knockout
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NF-kappa B / metabolism
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Receptors, Tumor Necrosis Factor / deficiency
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Receptors, Tumor Necrosis Factor / metabolism*
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Receptors, Tumor Necrosis Factor, Member 25
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Tumor Necrosis Factor-alpha / metabolism
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Tumor Necrosis Factors
Substances
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Apoptosis Regulatory Proteins
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Carrier Proteins
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Cytokine TWEAK
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Interleukin-8
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NF-kappa B
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Receptors, Tumor Necrosis Factor
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Receptors, Tumor Necrosis Factor, Member 25
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TNFRSF25 protein, human
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TNFSF12 protein, human
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Tnfrsf25 protein, mouse
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Tnfsf12 protein, mouse
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Tumor Necrosis Factor-alpha
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Tumor Necrosis Factors