Detection and clinical evaluation of GBV-C/HGV in plasma from patients with chronic hepatitis of unknown etiology

Clin Ter. 2000 Jul-Aug;151(4):241-5.

Abstract

Purpose: The role of GBV-C/HGV virus infection in patients with chronic hepatitis of unknown etiology has been studied. Two groups of patients have been included: a) 50 consecutive patients with chronic hepatitis (mean age 47 yrs; M/F = 35/15), negative for markers of hepatitis viruses (HBV-HCV). These patients did not show evidence of metabolic, autoimmune, alcoholic or toxic liver disease; b) 50 healthy blood donors (mean age 35 yrs; M/F = 35/15). All the subjects were HIV-seronegative.

Patients and methods: The detection of GBV-C/HGV RNA was performed by reverse transcription-polymerase chain reaction techniques and capillary zone electrophoresis analysis (CZE).

Results: There was no difference between the two groups of patients (14% vs 6%; p = 0.18 NS).

Conclusions: The etiopathogenetic role of GBV-C/HGV in chronic hepatitis of unknown etiology is yet to be shown.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Electrophoresis
  • Female
  • Flaviviridae / genetics
  • Flaviviridae / isolation & purification*
  • Hepatitis, Viral, Human / diagnosis*
  • Hepatitis, Viral, Human / virology*
  • Humans
  • Male
  • Middle Aged
  • RNA, Viral / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • RNA, Viral