Abstract
E2F/DP complexes play a pivotal role in the regulation of the G1/S transition in animals. Recently, plant E2F homologs have been cloned, but DP-related sequences have not been identified so far. Here we report that Arabidopsis thaliana contains at least two different DP-related genes, AtDPa and AtDPb. They exhibit an overall domain organization similar to that of their animal counterparts, although phylogenetic analysis demonstrated that they form a separate subgroup. AtDPs efficiently heterodimerize in vitro with the Arabidopsis E2F-related proteins, AtE2Fa and AtE2Fb through their dimerization domains. AtDPa and AtE2Fa are predominantly produced in actively dividing cells with highest transcript levels in early S phase cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Arabidopsis / genetics*
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Binding Sites
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Carrier Proteins*
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Cell Cycle
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Cell Cycle Proteins*
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DNA-Binding Proteins*
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Dimerization
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E2F Transcription Factors
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Gene Expression Regulation, Plant
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Genes, Plant / genetics*
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Humans
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Molecular Sequence Data
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Mutation / genetics
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Organ Specificity
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Phylogeny
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Precipitin Tests
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Protein Binding
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Protein Structure, Tertiary
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Retinoblastoma-Binding Protein 1
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Sequence Alignment
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Transcription Factor DP1
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Two-Hybrid System Techniques
Substances
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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DP transcription factor, plant
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E2F Transcription Factors
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Plant Proteins
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RNA, Messenger
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RNA, Plant
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Retinoblastoma-Binding Protein 1
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Transcription Factor DP1
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Transcription Factors
Associated data
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GENBANK/AJ294531
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GENBANK/AJ294532
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GENBANK/AJ294533
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GENBANK/AJ294534