Abstract
Several chemokines have been shown to act as angiogenic molecules or to modulate the activity of growth factors such as fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF). The detection of the CC chemokine receptor (CCR) 8 message in human umbilical vein endothelial cells (HUVECs) by reverse transcription- polymerase chain reaction (RT-PCR) and RNase protection assay (RPA), prompted us to investigate the potential role exerted by the CC chemokine I-309, a known ligand of such receptor, in both in vitro and in vivo angiogenesis assays. We show here that I-309 binds to endothelial cells, stimulates chemotaxis and invasion of these cells, and enhances HUVEC differentiation into capillary-like structures in an in vitro Matrigel assay. Furthermore, I-309 is an inducer of angiogenesis in vivo in both the rabbit cornea and the chick chorioallantoic membrane assay (CAM).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Allantois / blood supply
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Allantois / drug effects
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Animals
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Cell Differentiation / drug effects
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Cells, Cultured / drug effects
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Chemokine CCL1
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Chemokines, CC / metabolism
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Chemokines, CC / pharmacology*
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Chemotaxis / drug effects
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Chick Embryo
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Chorion / blood supply
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Chorion / drug effects
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Collagen
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Cornea / blood supply
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Cornea / drug effects
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Drug Combinations
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects*
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Gene Expression Regulation / drug effects
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Humans
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Laminin
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Male
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Neovascularization, Physiologic / drug effects*
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Proteoglycans
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Rabbits
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Receptors, CCR8
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Receptors, Chemokine / biosynthesis
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Receptors, Chemokine / genetics
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Umbilical Veins
Substances
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CCL1 protein, human
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CCR8 protein, human
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Chemokine CCL1
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Chemokines, CC
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Drug Combinations
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Laminin
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Proteoglycans
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RNA, Messenger
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Receptors, CCR8
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Receptors, Chemokine
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matrigel
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Collagen