Tc1, from Tityus cambridgei, is the first member of a new subfamily of scorpion toxin that blocks K(+)-channels

C V Batista; F Gómez-Lagunas; S Lucas; L D Possani

doi:10.1016/s0014-5793(00)02253-5

Tc1, from Tityus cambridgei, is the first member of a new subfamily of scorpion toxin that blocks K(+)-channels

FEBS Lett. 2000 Dec 8;486(2):117-20. doi: 10.1016/s0014-5793(00)02253-5.

Authors

C V Batista¹, F Gómez-Lagunas, S Lucas, L D Possani

Affiliation

¹ Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Mexico.

PMID: 11113450
DOI: 10.1016/s0014-5793(00)02253-5

Abstract

A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest known peptide from scorpion venom that recognizes K(+)-channels and constitutes a new structural subfamily of toxin, classified as alphaKTx 13.1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cell Line
Mice
Molecular Sequence Data
Neurotoxins / classification
Neurotoxins / isolation & purification
Neurotoxins / metabolism*
Peptides / classification
Peptides / isolation & purification
Peptides / metabolism*
Potassium Channel Blockers*
Potassium Channels / genetics
Scorpion Venoms / classification
Scorpion Venoms / isolation & purification
Scorpion Venoms / metabolism*
Scorpions / metabolism*
Shaker Superfamily of Potassium Channels
Spodoptera / cytology
Synaptosomes / metabolism

Substances

Neurotoxins
Peptides
Potassium Channel Blockers
Potassium Channels
Scorpion Venoms
Shaker Superfamily of Potassium Channels
tityustoxin