Cloning, expression, and characterization of cis-polyprenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus acidocaldarius

H Hemmi; S Yamashita; T Shimoyama; T Nakayama; T Nishino

doi:10.1128/JB.183.1.401-404.2001

Cloning, expression, and characterization of cis-polyprenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus acidocaldarius

J Bacteriol. 2001 Jan;183(1):401-4. doi: 10.1128/JB.183.1.401-404.2001.

Authors

H Hemmi¹, S Yamashita, T Shimoyama, T Nakayama, T Nishino

Affiliation

¹ Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba-yama 07, Sendai, Miyagi 980-8579, Japan.

Abstract

cis-polyprenyl diphosphate synthases are involved in the biosynthesis of the glycosyl carrier lipid in most organisms. However, only little is known about this enzyme of archaea. In this report, we isolated the gene of cis-polyprenyl diphosphate synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius, and characterized the recombinant enzyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkyl and Aryl Transferases / chemistry
Alkyl and Aryl Transferases / genetics*
Alkyl and Aryl Transferases / metabolism*
Amino Acid Sequence
Cloning, Molecular
Escherichia coli / enzymology
Escherichia coli / genetics
Molecular Sequence Data
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sequence Alignment
Substrate Specificity
Sulfolobus acidocaldarius / enzymology*
Sulfolobus acidocaldarius / genetics

Substances

Recombinant Proteins
Alkyl and Aryl Transferases
dehydrodolichyl diphosphate synthetase