Abstract
Extracellular nucleotides elicit multiple responses in eosinophils but no information on expression of purinergic receptors in these cells is available so far. In the present study we show that human eosinophils express the following P2Y and P2X subtypes: P2Y(1), P2Y(2), P2Y(4), P2Y(6), P2Y(11), and P2X(1), P2X(4), P2X(7), whose stimulation results in intracellular Ca(2+) increase and production of large amounts of reactive oxygen intermediates. These events are stimulated or inhibited, respectively, by P2 receptor agonists or antagonists.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / analogs & derivatives*
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
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Adenosine Triphosphate / analogs & derivatives*
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Adenosine Triphosphate / metabolism
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Adenosine Triphosphate / pharmacology
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Calcium / metabolism
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Chelating Agents / pharmacology
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Complement C5a / pharmacology
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Eosinophils / cytology
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Eosinophils / metabolism*
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Guanosine Triphosphate / metabolism
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Guanosine Triphosphate / pharmacology
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Humans
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Intracellular Fluid / metabolism
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Purinergic P2 Receptor Agonists
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Purinergic P2 Receptor Antagonists
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Reactive Oxygen Species / metabolism*
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Receptors, Purinergic P2 / chemistry*
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Receptors, Purinergic P2 / metabolism*
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Uridine Triphosphate / metabolism
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Uridine Triphosphate / pharmacology
Substances
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Chelating Agents
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Enzyme Inhibitors
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Purinergic P2 Receptor Agonists
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Purinergic P2 Receptor Antagonists
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Reactive Oxygen Species
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Receptors, Purinergic P2
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3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate
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2',3'-dialdehyde ATP
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KN 62
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Complement C5a
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Guanosine Triphosphate
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Adenosine Triphosphate
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Calcium
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Uridine Triphosphate