Generation and phenotype of cell lines derived from CF and non-CF mice that carry the H-2K(b)-tsA58 transgene

Am J Physiol Cell Physiol. 2001 Jan;280(1):C228-36. doi: 10.1152/ajpcell.2001.280.1.C228.

Abstract

Tracheal, renal, salivary, and pancreatic epithelial cells from cystic fibrosis [CF; cystic fibrosis transmembrane conductance regulator (CFTR) -/-] and non-CF mice that carry a temperature-sensitive SV40 large T antigen oncogene (ImmortoMouse) were isolated and maintained in culture under permissive conditions (33 degrees C with interferon-gamma). The resultant cell lines have been in culture for >1 year and 50 passages. Each of the eight cell lines form polarized epithelial barriers and exhibit regulated, electrogenic ion transport. The four non-CF cell lines (mTEC1, mCT1, mSEC1, and mPEC1) express cAMP-regulated Cl(-) permeability and cAMP-stimulated Cl(-) secretion. In contrast, the four CFTR -/- cell lines (mTEC1-CF, mCT1-CF, mSEC1-CF, and mPEC1-CF) each lack cAMP-stimulated Cl(-) secretory responses. Ca(2+)-activated Cl(-) secretion is retained in both CF and non-CF cell lines. Thus we have generated genetically well-matched epithelial cell lines from several tissues relevant to cystic fibrosis that either completely lack CFTR or express endogenous levels of CFTR. These cell lines should prove useful for studies of regulation of epithelial cell function and the role of CFTR in cell physiology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Line, Transformed / cytology
  • Cell Line, Transformed / drug effects
  • Cell Line, Transformed / metabolism*
  • Cell Membrane Permeability / drug effects
  • Cell Membrane Permeability / genetics
  • Chloride Channels / drug effects
  • Chloride Channels / genetics
  • Chloride Channels / metabolism
  • Culture Media / pharmacology
  • Cyclic AMP / metabolism
  • Cyclic AMP / pharmacology
  • Cystic Fibrosis / genetics*
  • Cystic Fibrosis / metabolism
  • Cystic Fibrosis / physiopathology
  • Cystic Fibrosis Transmembrane Conductance Regulator / drug effects
  • Cystic Fibrosis Transmembrane Conductance Regulator / genetics
  • Cystic Fibrosis Transmembrane Conductance Regulator / metabolism
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism*
  • Genotype
  • H-2 Antigens / genetics*
  • H-2 Antigens / metabolism
  • Ion Transport / drug effects
  • Ion Transport / physiology
  • Male
  • Mice
  • Mice, Inbred CFTR / anatomy & histology
  • Mice, Inbred CFTR / genetics*
  • Mice, Inbred CFTR / metabolism
  • Phenotype
  • Transgenes / physiology

Substances

  • Chloride Channels
  • Culture Media
  • H-2 Antigens
  • H-2Kb protein, mouse
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Cyclic AMP