Myeloma proteins that bind Hsp65 (GroEL) are polyreactive and are found in high incidence in pristine induced plasmacytomas

Curr Top Microbiol Immunol. 2000:252:265-71. doi: 10.1007/978-3-642-57284-5_27.

Abstract

The myeloma proteins produced by 44 plasmacytomas (PCTs) recently induced by pristane in BALB/cAnPt and closely related PCT susceptible congenic strains of mice were isolated chromatographically and screened against a panel of 10 protein, nucleic acid and lipid antigens. This sample was highly unusual because 82% of the proteins had IgG isotopes. Nine of the proteins bound to Hsp65 (GroEL), and all of these were polyreactative. Twenty-one of the myeloma proteins were polyreactive and bound two or more antigens in the panel, and five were monoreactive. Thus, an antigen binding activity was determined for 59% of these myeloma proteins.

Publication types

  • Review

MeSH terms

  • Animals
  • Antibody Specificity
  • Antigen-Antibody Reactions
  • Arthritis / chemically induced
  • Arthritis / immunology
  • Ascitic Fluid / chemistry
  • Bacterial Proteins*
  • Chaperonin 60 / immunology*
  • Chaperonin 60 / metabolism
  • Chaperonins / immunology*
  • Chaperonins / metabolism
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Heavy Chains / immunology
  • Mice
  • Mice, Inbred BALB C
  • Myeloma Proteins / immunology*
  • Myeloma Proteins / metabolism
  • Plasmacytoma / chemically induced
  • Plasmacytoma / immunology*
  • Terpenes / toxicity*

Substances

  • Bacterial Proteins
  • Chaperonin 60
  • Immunoglobulin Heavy Chains
  • Myeloma Proteins
  • Terpenes
  • heat-shock protein 65, Mycobacterium
  • pristane
  • Chaperonins