Molecular characterization of a Cryptosporidium isolate from a black bear

J Parasitol. 2000 Oct;86(5):1166-70. doi: 10.1645/0022-3395(2000)086[1166:MCOACI]2.0.CO;2.

Abstract

To further validate the observation of the existence of host-adapted strains of Cryptosporidium parvum, we genetically characterized an isolate of Cryptosporidium parasite from a black bear. Sequence analysis of the ribosomal RNA small subunit and the 70-kDa heat shock protein (HSP70) showed that this parasite represents a new genotype of C. parvum and is related to the C. parvum dog genotype. This finding is helpful for clarifying Cryptosporidium taxonomy.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cattle
  • Cryptosporidiosis / parasitology
  • Cryptosporidiosis / veterinary*
  • Cryptosporidium parvum / classification*
  • Cryptosporidium parvum / genetics*
  • Dogs
  • Genes, rRNA / genetics
  • HSP70 Heat-Shock Proteins / genetics
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal / genetics
  • Sequence Analysis, DNA
  • Ursidae / parasitology*

Substances

  • HSP70 Heat-Shock Proteins
  • RNA, Ribosomal

Associated data

  • GENBANK/AF247535
  • GENBANK/AF247536