A Schistosoma mansoni 62-kDa band is identified as an irradiated vaccine T-cell antigen and characterized as calreticulin

J Parasitol. 2000 Oct;86(5):993-1000. doi: 10.1645/0022-3395(2000)086[0993:ASMKBI]2.0.CO;2.

Abstract

The Schistosoma mansoni soluble adult worm antigen (SAWA) bands of 62/60 kDa were found to contain immunodominant T-cell immunogen(s) in irradiated cercariae-immunized Swiss and C57BL/6 mice. In the present study, spleen T cells of BALB/c mice immunized twice with ultraviolet light-irradiated cercariae proliferated and produced interleukin 4 in response to the 62/60-kDa SAWA bands in T-cell western assays. To characterize the 62/60-kDa bands, an adult S. mansoni worm cDNA expression library constructed in lambdagt11 was immunoscreened with serum of mice immunized with the 62/60-kDa antigens, and the immunoreactive cDNA inserts were sequenced. Purified 62- and 60-kDa proteins were used for amino acid microsequencing and for immunization studies in Swiss, C57BL/6, and BALB/c mice and rabbits. Taken together, the data indicated that the 60-kDa molecules are poorly immunogenic in mice and rabbits, whereas the 62-kDa species identified as S. mansoni calreticulin, is a good T- and B-cell antigen and represents a potential vaccine candidate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Helminth / chemistry
  • Antigens, Helminth / genetics
  • Antigens, Helminth / immunology*
  • B-Lymphocytes / immunology
  • Blotting, Western
  • Calcium-Binding Proteins / chemistry
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / immunology*
  • Calreticulin
  • Cloning, Molecular
  • Epitopes, T-Lymphocyte / genetics
  • Epitopes, T-Lymphocyte / immunology*
  • Female
  • Immunization / methods
  • Immunodominant Epitopes / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Rabbits
  • Ribonucleoproteins / chemistry
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / immunology*
  • Schistosoma mansoni / growth & development
  • Schistosoma mansoni / immunology*
  • Schistosomiasis mansoni / immunology
  • Schistosomiasis mansoni / parasitology
  • T-Lymphocytes / immunology*
  • Ultraviolet Rays
  • Vaccines / immunology

Substances

  • Antigens, Helminth
  • Calcium-Binding Proteins
  • Calreticulin
  • Epitopes, T-Lymphocyte
  • Immunodominant Epitopes
  • Ribonucleoproteins
  • Vaccines