Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune

K Scholtmeijer; H A Wösten; J Springer; J G Wessels

doi:10.1128/AEM.67.1.481-483.2001

Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune

Appl Environ Microbiol. 2001 Jan;67(1):481-3. doi: 10.1128/AEM.67.1.481-483.2001.

Authors

K Scholtmeijer¹, H A Wösten, J Springer, J G Wessels

Affiliation

¹ Molecular Plant Biology Laboratory, Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands. [email protected]

Abstract

Previously, it was shown that introns are required for efficient mRNA accumulation in Schizophyllum commune and that the presence of AT-rich sequences in the coding region of genes can result in truncation of transcripts in this homobasidiomycete. Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AT Rich Sequence / genetics*
Anti-Bacterial Agents / pharmacology*
Base Sequence
Drug Resistance, Microbial / genetics
Hygromycin B / pharmacology*
Introns / genetics*
Molecular Sequence Data
RNA, Fungal / genetics
RNA, Fungal / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism*
Schizophyllum / drug effects*
Schizophyllum / genetics*

Substances

Anti-Bacterial Agents
RNA, Fungal
RNA, Messenger
Hygromycin B