Fission yeast homolog of murine Int-6 protein, encoded by mouse mammary tumor virus integration site, is associated with the conserved core subunits of eukaryotic translation initiation factor 3

J Biol Chem. 2001 Mar 30;276(13):10056-62. doi: 10.1074/jbc.M010188200. Epub 2000 Dec 27.

Abstract

The murine int-6 locus, identified as a frequent integration site of mouse mammary tumor viruses, encodes the 48-kDa eIF3e subunit of translation initiation factor eIF3. Previous studies indicated that the catalytically active core of budding yeast eIF3 consists of five subunits, all conserved in eukaryotes, but does not contain a protein closely related to eIF3e/Int-6. Whereas the budding yeast genome does not encode a protein closely related to murine Int-6, fission yeast does encode an Int-6 ortholog, designated here Int6. We found that fission yeast Int6/eIF3e is a cytoplasmic protein associated with 40 S ribosomes. FLAG epitope-tagged Tif35, a putative core eIF3g subunit, copurified with Int6 and all five orthologs of core eIF3 subunits. An int6 deletion (int6Delta) mutant was viable but grew slowly in minimal medium. This slow growth phenotype was accompanied by a reduction in the amount of polyribosomes engaged in translation and was complemented by expression of human Int-6 protein. These findings support the idea that human and Schizosaccharomyces pombe Int-6 homologs are involved in translation. Interestingly, haploid int6Delta cells showed unequal nuclear partitioning, possibly because of a defect in tubulin function, and diploid int6Delta cells formed abnormal spores. We propose that Int6 is not an essential subunit of eIF3 but might be involved in regulating the activity of eIF3 for translation of specific mRNAs in S. pombe.

MeSH terms

  • Animals
  • Binding Sites
  • Conserved Sequence
  • Cytoplasm / metabolism
  • Epitopes
  • Eukaryotic Initiation Factor-3
  • Gene Deletion
  • Humans
  • Mammary Tumor Virus, Mouse / genetics
  • Mass Spectrometry
  • Mice
  • Mutation
  • Peptide Initiation Factors / chemistry*
  • Peptide Initiation Factors / genetics
  • Phenotype
  • Plasmids / metabolism
  • Polyribosomes / metabolism
  • Precipitin Tests
  • Prokaryotic Initiation Factor-3
  • Protein Binding
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins / chemistry
  • Proto-Oncogene Proteins / genetics*
  • RNA, Messenger / metabolism
  • Ribosomes / metabolism
  • Saccharomyces cerevisiae / metabolism
  • Schizosaccharomyces / chemistry
  • Schizosaccharomyces / genetics*
  • Tubulin / metabolism

Substances

  • Epitopes
  • Eukaryotic Initiation Factor-3
  • Peptide Initiation Factors
  • Prokaryotic Initiation Factor-3
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Tubulin