A novel monoclonal antibody specific for the N-terminal end of GAD65

J Neuroimmunol. 2001 Feb 1;113(1):63-71. doi: 10.1016/s0165-5728(00)00423-9.

Abstract

We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity*
  • Autoantibodies / immunology
  • Blotting, Western
  • DNA, Complementary
  • Diabetes Mellitus, Type 1 / enzymology
  • Diabetes Mellitus, Type 1 / immunology
  • Glutamate Decarboxylase / chemistry
  • Glutamate Decarboxylase / immunology*
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Heavy Chains / immunology
  • Immunoglobulin Light Chains / genetics
  • Immunoglobulin Light Chains / immunology
  • Immunohistochemistry
  • Isoenzymes / chemistry
  • Isoenzymes / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Radioimmunoassay
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Antibodies, Monoclonal
  • Autoantibodies
  • DNA, Complementary
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Isoenzymes
  • Glutamate Decarboxylase
  • glutamate decarboxylase 2