[Tumor M2 pyruvate kinase in renal cell carcinoma. Studies of plasma in patients]

Urologe A. 2000 Nov;39(6):554-6. doi: 10.1007/s001200050410.
[Article in German]

Abstract

Tumor cell metabolism is characterized by a high rate of aerobic glycolysis. The metabolic differences require changes in glycolytic enzyme activities and isoenzyme patterns. The inactive form of the M2 pyruvate kinase (Tu M2-PK) is specifically expressed in tumor cells and has been detected immunohistochemically in tumor tissue but also in peripheral blood of patients with different malignant tumors. In this study, Tu M2-PK in the plasma of patients with renal cell carcinoma (RCC) was compared with healthy volunteers. Tu M2-PK was quantified with a commercially available enzyme linked immunosorbent assay (ELISA) kit. Using the ELISA kit, plasma probes of 57 healthy individuals were compared to 63 patients with RCC (51 patients with non-metastatic RCC, 12 patients with metastatic RCC). Statistical analysis was performed with the non-parametric ANOVA test according to Kruskal-Wallis. In patients with renal cell carcinoma, Tu M2-PK was significantly higher than in healthy volunteers. For organ-defined, non-metastatic tumors, sensitivity was only 27.5%, if the 95% reference values of the control group were used for discrimination. The differences were more pronounced in patients with metastatic disease. Tu M2-PK was significantly enhanced compared to healthy controls, but also to the group with non-metastatic disease, the sensitivity was 66.7%. Our data show that Tu M2-PK has no impact as an unspecific marker for the diagnosis of renal cell carcinoma. This is especially relevant to organ-defined, non-metastatic RCC. In advanced metastatic disease, a potential importance as a parameter for treatment control in palliative therapeutic approaches can be assumed, and warrants further investigations.

Publication types

  • English Abstract

MeSH terms

  • Biomarkers, Tumor / blood*
  • Carcinoma, Renal Cell / diagnosis*
  • Carcinoma, Renal Cell / enzymology
  • Carcinoma, Renal Cell / pathology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Kidney Neoplasms / diagnosis*
  • Kidney Neoplasms / enzymology
  • Kidney Neoplasms / pathology
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Predictive Value of Tests
  • Pyruvate Kinase / blood*

Substances

  • Biomarkers, Tumor
  • Pyruvate Kinase